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Item #:
85746627
Corning Matrigel Basement Membrane Matrix,LDEV-Free, 10mL
Item #:
85746627
CHF 630
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Corning Matrigel matrix is a solubilized basement membrane preparation rich in ECM proteins and growth factors.
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What Stands Out
LDEV-Free Formula
This Matrigel is specifically designed to be LDEV-free, reducing contamination risk in cell culture and enhancing reproducibility in research applications.
Versatile Applications
Ideal for a broad range of cell types and applications, including 3D cell culture and tissue engineering, providing researchers with flexibility and reliability in their experimental setups.
Easy Handling
Delivered in a convenient 10mL volume, this product simplifies the process of preparation and application, saving time and reducing waste for laboratories focused on efficiency.
Product Details
| Item Weight | 0.03 lbs (10 grams) |
Who Should Buy?
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Cell Culture Research
Ideal for researchers looking for a consistent extracellular matrix for 3D cell culture studies and assays.
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Tissue Engineering
Beneficial for scientists in tissue engineering applications requiring a supportive scaffold for cell growth and differentiation.
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Stem Cell Applications
Suitable for stem cell researchers needing a reliable matrix to facilitate proper cell attachment and growth.
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Non-Cell Experiments
Not suitable for assays or experiments that do not involve live cellular components or tissue systems.
Product Description
Corning Matrigel Basement Membrane Matrix,LDEV-Free, 10mL
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Customer Questions & Answers
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Question:
What is Corning Matrigel Basement Membrane Matrix, LDEV-Free?
Answer: Corning Matrigel Basement Membrane Matrix, LDEV-Free is a proprietary mixture of basement membrane proteins designed for the culture of various cell types. Unlike standard Matrigel, LDEV-Free is specifically formulated to reduce the risk of contamination from murine pathogens. This makes it particularly suitable for applications in stem cell research, tissue engineering, and regenerative medicine where a clean and supportive environment for cell growth is crucial. -
Question:
What applications can I use LDEV-Free Matrigel for?
Answer: LDEV-Free Matrigel is widely used in various applications such as 3D cell culture, organoid formation, drug discovery, and bioengineering. Researchers can utilize it to promote cell differentiation and growth in a controlled environment. Ideal for studies involving epithelial cells, endothelial cells, and various stem cell types, it's an essential component in laboratory setups that aim to replicate in vivo conditions. -
Question:
How do I prepare Corning Matrigel for use?
Answer: To prepare Corning Matrigel for use, it is essential to thaw the matrix on ice and then gently mix it to ensure an even consistency. After thawing, you can dilute it in your specific culture medium at the desired concentration, often between 50-500 µg/mL, depending on your application. This preparation allows for optimal cell attachment and morphology, making it vital to follow specific experimental protocols to achieve effective results. -
Question:
Is Corning Matrigel LDEV-Free suitable for primary cell cultures?
Answer: Yes, Corning Matrigel LDEV-Free is highly suitable for primary cell cultures. Its lack of murine pathogens ensures a safer environment for sensitive cell types. This matrix supports the growth and maintenance of primary cells by mimicking the extracellular environment, which is essential for cell viability and function in experimentation. Furthermore, its ability to promote normal cell behavior makes it a popular choice among researchers. -
Question:
What are the storage conditions for Corning Matrigel?
Answer: Corning Matrigel Basement Membrane Matrix should be stored at -20°C to maintain its integrity and functionality. Avoid repeated freezing and thawing cycles, as this can degrade its quality. For optimal performance, it's recommended to thaw only the amount needed for specific experiments and to keep the remainder stored frozen until needed. By following these storage guidelines, you help preserve the matrix's effectiveness for future uses. -
Question:
Can I use Corning Matrigel for transplanting cell cultures?
Answer: Absolutely! Corning Matrigel can be utilized for transplanting cell cultures, especially when working with stem cells and organoids. Its biocompatibility and supportive properties facilitate the integration of transplanted cells into new environments, promoting better survival and function post-transplant. This is particularly useful in regenerative medicine applications, including tissue repair and organ regeneration studies. -
Question:
How does Corning LDEV-Free Matrigel impact cell differentiation?
Answer: Corning LDEV-Free Matrigel positively impacts cell differentiation by providing a conducive extracellular matrix environment that mimics natural tissue. The various proteins present in Matrigel activate specific signaling pathways that promote the maturation of stem cells into desired cell types. This capability is essential for studies exploring differentiation mechanisms in cell biology, especially in regenerative and developmental research. -
Question:
What precautions should I take when handling Matrigel?
Answer: When handling Matrigel, it's crucial to use personal protective equipment such as gloves and goggles to maintain a sterile environment. Work in a sterile hood to prevent contamination, and utilize sterile pipettes and tubes to manipulate the matrix. Additionally, avoid exposing Matrigel to excessive heat or light, as this can affect its properties. Following these precautions will help ensure reliable and reproducible experimental outcomes. -
Question:
What cell types can thrive in LDEV-Free Matrigel?
Answer: LDEV-Free Matrigel supports a wide range of cell types, including epithelial, endothelial, and various stem cells. This versatility makes it suitable for applications in tissue engineering and regenerative medicine. Researchers often find success with cancer cell lines, primary cells, and induced pluripotent stem cells, as they benefit from the matrix’s ability to mimic the natural extracellular environment and provide necessary biochemical cues for growth. -
Question:
Where can I buy Corning Matrigel Basement Membrane Matrix, LDEV-Free, 10mL?
Answer: You can purchase Corning Matrigel Basement Membrane Matrix, LDEV-Free, 10mL on Ubuy. They offer a broad selection of research and laboratory supplies, ensuring you can find the right products for your scientific needs with ease, efficiency, and accessibility.
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CHF 630
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Features & Benefits
- Extracted from Engelbreth-Holm-Swarm (EHS) mouse sarcoma
- Contains Laminin, Collagen IV, heparan sulfate proteoglycans, and entactin/nidogen
- Extensive PCR testing for pathogens
- Protein concentrations determined by Lowry method
- Size: 10ml (Also available in 5ml version)
- Corning P/N: 354234
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